rabbit polyclonal antibody against ddr2  (GeneTex)

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Representative image of DDR2 and collagen type I immunostaining in human breast cancer. ( A – C ): immunostaining of DDR2 in breast cancer cells ( A ), normal breast epithelium ( B ), and human heart as a positive control of DDR2 ( C ). ( D – F ): immunostaining of collagen type I in cancerous stroma ( D ), normal breast stroma ( E ), and human skin as a positive control of collagen type I ( F ). Bar = 100 µm, respectively.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Immunostaining, Positive Control

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinicopathological factors in 224 breast carcinomas.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinicopathological factors according to collagen type I status in 224 breast carcinomas.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Association between DDR2 status and clinical outcomes of breast cancer patients (n = 224). ( A – D ): disease-free survival ( A , C ) and breast cancer-specific survival ( B , D ) according to DDR2 status ( A , B ) or DDR2/collagen type I combination status ( C , D ). ( E – H ): disease-free survival according to DDR2 status ( E , F ) or DDR2/collagen type I combination status ( G , H ) in the patients who received chemotherapy ( E , G ) or not ( F , H ).

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: Uni- and multivariate analysis of disease-free survival.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 in the proliferation of human breast cancer cell lines in the presence of collagen type I. ( A ) Immunoblotting of exogenous DDR2 protein in MCF-7, MDA-MB-231, and T47D cells. ( B – D ): cell proliferation of MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( D ) transfected with an empty vector or DDR2-expressing vector in the absence or presence of collagen coating. ( E ) Immunoblotting of DDR2 in the cells transfected with siRNA against DDR2 (siDDR2-1, 2). ( F – H ): cell proliferation of MCF-7 ( F ), MDA-MB-231 ( G ), and T47D ( H ) transfected with siRNAs in the presence of collagen coating. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the empty vector, respectively.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 on the resistance to epirubicin in the breast cancer cell lines. ( A – C ): viability of MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( C ) transfected with an empty vector or DDR2-expressing vector under epirubicin treatment (500 nM for MCF-7; 250 nM for MDA-MB-231 and T47D). These cells were plated onto collagen-coated culture plates. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the empty vector, respectively. ( D – F ): viability of MCF-7 ( D ), MDA-MB-231 ( E ), and T47D ( F ) transfected with siRNA targeting DDR2 under epirubicin treatment. ( G , H ) mRNA and protein expression in chemosensitive parental cells and epirubicin-resistant cells ( G ; MCF-7 series, H ; MDA-MB-231 series). ** p < 0.01, respectively. ( I , J ) The effect of DDR2 inhibitor WRG-28 treatment (48 h) on the proliferation of chemosensitive parental cells and epirubicin-resistant cells ( F ; MCF-7 series, G ; MDA-MB-231 series).

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques: Transfection, Plasmid Preparation, Expressing

Journal: Cancers

Article Title: Discoidin Domain Receptor 2 Contributes to Breast Cancer Progression and Chemoresistance by Interacting with Collagen Type I

doi: 10.3390/cancers16244285

Figure Lengend Snippet: The effect of DDR2 on the apoptosis of breast MCF-7 ( A ), MDA-MB-231 ( B ), and T47D ( C ). *** p < 0.001.

Article Snippet: The rabbit polyclonal antibody against DDR2 was purchased from GeneTex (Irvine, CA, USA).

Techniques:

Journal: Scientific Reports

Article Title: Integrated Molecular Profiling of Human Gastric Cancer Identifies DDR2 as a Potential Regulator of Peritoneal Dissemination

doi: 10.1038/srep22371

Figure Lengend Snippet: ( a ) EEM-deduced activity profiles of GDES and other gene sets were evaluated by clustering analysis. ( b ) Kaplan–Meier survival curves for the 198 patients in the Singaporean cohort, with classification based on DDR2 mRNA expression levels measured using a microarray. ( c ) DDR2 expression was significantly higher in gastric cancer patients with peritoneal metastasis (Singaporean cohort). ( d ) Kaplan–Meier survival curves for the 195 patients in the Japanese cohort, as measured by qRT-PCR. ( e ) High DDR2 expression associated with gastric cancer patients that developed peritoneal metastasis, as determined by performing a Pearson’s chi-square test (Japanese cohort).

Article Snippet: For antigen retrieval, tissue sections were deparaffinized and boiled in 0.01 M sodium citrate buffer in a microwave for 10 minutes at 500 W. A rabbit polyclonal antibody against DDR2 (H-108, 1:100 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) and a mouse monoclonal antibody against E-cadherin (36/E-Cadherin, 1:100 dilution; BD Bioscience) were used as primary antibodies.

Techniques: Activity Assay, Expressing, Microarray, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Integrated Molecular Profiling of Human Gastric Cancer Identifies DDR2 as a Potential Regulator of Peritoneal Dissemination

doi: 10.1038/srep22371

Figure Lengend Snippet: ( a ) Correlation plot between DDR2 expression and the fraction of methylation at the promoter region of DDR2 in 173 gastric cancer samples from the Singaporean cohort. ( b ) Box plot of the fraction of DDR2 promoter methylation between patients, with or without peritoneal metastasis. ( c ) Representative images of DDR2 and E-cadherin protein expression in gastric cancer cells. Scale bars, 100 μm. ( d) DDR2 and E-cadherin expression were inversely correlated by immunohistochemical analysis of 76 gastric cancer samples.

Article Snippet: For antigen retrieval, tissue sections were deparaffinized and boiled in 0.01 M sodium citrate buffer in a microwave for 10 minutes at 500 W. A rabbit polyclonal antibody against DDR2 (H-108, 1:100 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) and a mouse monoclonal antibody against E-cadherin (36/E-Cadherin, 1:100 dilution; BD Bioscience) were used as primary antibodies.

Techniques: Expressing, Methylation, Immunohistochemical staining

Journal: Scientific Reports

Article Title: Integrated Molecular Profiling of Human Gastric Cancer Identifies DDR2 as a Potential Regulator of Peritoneal Dissemination

doi: 10.1038/srep22371

Figure Lengend Snippet: ( a ) DDR2 expression was suppressed in 58As9Luc cells by 2 independent shRNAs. ( b ) DDR2 knockdown resulted in lower cell proliferation on collagen I-coated 96-well plates, but did not affect cell proliferation on normal plates. ( c , d ) Effects of DDR2 on ( c ) cancer cell migration and ( d ) invasion. ( e ) DDR2-knockdown 58As9Luc cells were orthotopically transplanted into the stomach walls of nude mice. ( f , g ) The normalized weight of gastric wall tumors ( f ) and the number of disseminated metastatic tumors ( g ) at 28 days post-transplantation with 58As9Luc cells with silenced DDR2 expression. Control, DDR2 sh#1, and sh#2: n = 8. *p < 0.05, **p < 0.01.

Article Snippet: For antigen retrieval, tissue sections were deparaffinized and boiled in 0.01 M sodium citrate buffer in a microwave for 10 minutes at 500 W. A rabbit polyclonal antibody against DDR2 (H-108, 1:100 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) and a mouse monoclonal antibody against E-cadherin (36/E-Cadherin, 1:100 dilution; BD Bioscience) were used as primary antibodies.

Techniques: Expressing, Knockdown, Migration, Transplantation Assay, Control